US B-2 bombers attacked weapons depots belonging to Houthi rebels in Yemen.
Defense Secretary Lloyd Austin said the operation was ordered late Wednesday by President Joe Biden to "further degrade" the Houthis' ability to launch attacks on commercial and military ships in the Red Sea.
Austin did not mention Iran by name, but he said the use of the B-2 bomber, which is the only fighter jet in the US arsenal that can carry so-called bunker-busting bombs.
In Yemen, the Houthis have sporadically attacked ships in the Red Sea, a major trade route, for years, but attacks against civilian tankers and cargo ships have intensified since the conflict between Israel and Hamas began last year.
The Houthis say their attacks are a sign of solidarity with Hamas, which is also supported financially and with weapons by Iran, and Palestinians in general.